A stability-indicating LC method for the simultaneous Determination of Levocetirizine dihydrochloride and Pseudoephedrine sulfate in Tablet dosage forms

A Stability indicating reverse phase high performance liquid chromatographic method has been developed for the simultaneous determination of Levocetirizine dihydrochloride and Pseudoephedrine sulfate in pharmaceutical formulation. The mobile phase A consisted of Potassium dihydrogen phosphate Buffer 0.05M and 1-Ocatne sulphonic acid sodium salt 0.25%, pH adjusted to 3.0 with orthophospheric acid. Mobile Phase B: Acetonitrile, Gradient elution at flow rate of 1 mL/min and Column temperature at 40C. Detector wavelength of 242 nm using a photodiode array detector. The described method shows excellent linearity over a range of 200–10 μg ml for Levocetirizine dihydrochloride and 7200-360 μg ml for Pseudoephedrine sulfate. The correlation coefficient for Levocetirizine dihydrochloride and Pseudoephedrine sulfate are 0.9999. The proposed method was found to be suitable, accurate for quantitative determination and the stability study of Levocetirizine dihydrochloride and Pseudoephedrine sulfate in pharmaceutical preparations.